Zinc–rs13266634 and the Arrival of Diabetes Pharmacogenetics: The “Zinc Mystique”

Advances in our understanding of the genetics of type 2 diabetes have been astronomical over the past decade with more than 100 single nucleotide polymorphisms (SNPs) associated with modest increases in diabetes risk and differences in related traits such as fasting glucose (1,2). However, these SNPs have contributed more to elucidating biologic pathways than predicting diabetes risk (3). Nonetheless, the most promising work suggests that we are on the cusp of being able to use genetic information to individualize treatment for type 2 diabetes prevention and management (4,5). The promise of pharmacogenetics is predicated on the existence of known individual variation in response to interventions and the strong biologic basis for genetic variation as a primary, immutable difference between individuals. rs13266634 is one of the most consistently replicated diabetes risk variants (odds ratio of 1.14 for the R allele) (6). This SNP encodes a C→T base substitution in the SLC30A8 gene, resulting in a change in the coded protein (Arg325Trp or R325W). The frequency of the diabetes risk R allele is 91.5%, 71.7%, and 56.7% in Africans, Europeans, and Asians, respectively (1,000 Genomes). The SLC30A8 gene encodes zinc transporter-8 (ZnT8). This transporter is specific to pancreatic islets and is mainly expressed in b-cells that transport zinc from the cytoplasm into insulin secretory vesicles (7). This zinc stabilizes insulin hexamers, prevents insulin degradation, and is cosecreted with insulin, proinsulin, and C-peptide during the early stage of glucose-stimulated insulin secretion (GSIS) (7,8). Cosecreted zinc also passes through the portal vein with the other contents of the insulin secretory vesicle and appears to inhibit hepatic clearance of insulin (8). Although preclinical studies consistently show structural abnormalities of insulin secretory vesicles in the absence of ZnT8 (9–11), they are not consistent regarding the impact of ZnT8 abnormalities on GSIS (9–12). The few human studies that evaluated quantitative traits suggest that carriers of the R risk allele have abnormalities in both GSIS and insulin processing (13–16). In this issue, Shan et al. (17) suggest that plasma zinc concentrations are associated with diabetes in a genotypespecific fashion, a finding that highlights the potential of rs13266634 as a strong genetic candidate for individualizing diabetes care. The authors first measured zinc levels in 1,776 Chinese people with normal glucose tolerance (n = 793), newly diagnosed impaired glucose tolerance/ impaired fasting glucose (n = 218), and newly diagnosed type 2 diabetes (n = 785) and observed lower plasma zinc concentrations in those with diabetes. The novel finding, however, was a statistical interaction between zinc levels and rs13266634 such that the benefit of higher zinc levels on the odds of diabetes decreased with increasing copies of the R (risk) allele (WW.RW.RR). Shan et al. provide the first published data on the potential interaction between zinc levels and rs13266634 on diabetes risk. A major strength of the new report is its focus on a biologically plausible interaction: rs13266634 is a coding variant that affects a specific zinc transporter that is directly involved in insulin secretion in the b-cell. One could therefore hypothesize that the impact of available zinc would vary across its genotype. In fact, these findings are consistent with a prior meta-analysis of five cross-sectional studies that showed higher zinc intake seemed to reduce the fasting glucose-raising effect of rs11558471; rs11558471 is an SLC30A8 SNP in tight linkage disequilibrium with rs13266634 (18). Of course, plasma zinc levels are unlikely to reflect zinc exposure at the level of the b-cell, and zinc levels assessed at a single point in time may not reflect